investigation of the effect of substrate conditions on electron transfer of glucose oxidase

direct electron transferring of glucose oxidase was investigated   on reduced graphene and graphene oxide templates. the direct electrochemistry   glucose oxidase on   graphene showed a cyclic voltammograms corresponding to the fad/fadh 2   redox couple with an anodic, cathodic and formal potential of -430, -460 and   -445 mv, respectively in 0.1 m phosphate buffer solution and air saturated   condition for   similarity of in vivo usage. the cyclic voltammograms of glucose oxidase on   graphene is reversible. also, the voltammograms results show, the current   intensity of glucose oxidase on graphene is high, due to fast electron   transferring. moreover, the linear rang concentration of glucose on graphene   are 0.4–9 µm. these studies make useful insight into the enzyme   immobilization on nanoparticles for biosensors and bio-fuel cell   preparation.